human crth2 microbead kit Search Results


crth2 microbead kit  (Miltenyi Biotec)
94
Miltenyi Biotec crth2 microbead kit
( A – H ) hILC2s (CD45 + , lineage – , <t>CRTH2</t> + , and CD127 + ) were freshly isolated from PBMCs of healthy donors and cultured with or without anti-ICOS ligand (anti-ICOSL). Right panel shows hILC2 purity after being sorted. ( B – F ) Levels of IL-10 ( B ), IL-4 ( C ), IL-5 ( D ), IL-6 ( E ), and IL-13 ( F ) in the culture supernatants following treatment with or without anti-ICOSL. n = 6. ( G and H ) The expression levels of intranuclear Ki67 ( G ) and GATA-3 ( H ) expression is presented as MFI. n = 6. ( I and J ) Representative histogram of MAF ( I ) and NFIL3 ( J ) protein expression levels . Corresponding quantitation is presented as MFI. n = 4. Data are presented as mean ± SEM. Two-tailed Student’s t test was employed for statistical analysis; * P < 0.05, ** P < 0.01, and *** P < 0.001. Schematic images were created in Adobe Illustrator. FMO, fluorescence minus one.
Crth2 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
crth2 microbead kit - by Bioz Stars, 2026-03
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( A – H ) hILC2s (CD45 + , lineage – , CRTH2 + , and CD127 + ) were freshly isolated from PBMCs of healthy donors and cultured with or without anti-ICOS ligand (anti-ICOSL). Right panel shows hILC2 purity after being sorted. ( B – F ) Levels of IL-10 ( B ), IL-4 ( C ), IL-5 ( D ), IL-6 ( E ), and IL-13 ( F ) in the culture supernatants following treatment with or without anti-ICOSL. n = 6. ( G and H ) The expression levels of intranuclear Ki67 ( G ) and GATA-3 ( H ) expression is presented as MFI. n = 6. ( I and J ) Representative histogram of MAF ( I ) and NFIL3 ( J ) protein expression levels . Corresponding quantitation is presented as MFI. n = 4. Data are presented as mean ± SEM. Two-tailed Student’s t test was employed for statistical analysis; * P < 0.05, ** P < 0.01, and *** P < 0.001. Schematic images were created in Adobe Illustrator. FMO, fluorescence minus one.

Journal: The Journal of Clinical Investigation

Article Title: ICOS regulates IL-10 production in group 2 innate lymphoid cells via cholesterol and cortisol biosynthesis

doi: 10.1172/JCI193134

Figure Lengend Snippet: ( A – H ) hILC2s (CD45 + , lineage – , CRTH2 + , and CD127 + ) were freshly isolated from PBMCs of healthy donors and cultured with or without anti-ICOS ligand (anti-ICOSL). Right panel shows hILC2 purity after being sorted. ( B – F ) Levels of IL-10 ( B ), IL-4 ( C ), IL-5 ( D ), IL-6 ( E ), and IL-13 ( F ) in the culture supernatants following treatment with or without anti-ICOSL. n = 6. ( G and H ) The expression levels of intranuclear Ki67 ( G ) and GATA-3 ( H ) expression is presented as MFI. n = 6. ( I and J ) Representative histogram of MAF ( I ) and NFIL3 ( J ) protein expression levels . Corresponding quantitation is presented as MFI. n = 4. Data are presented as mean ± SEM. Two-tailed Student’s t test was employed for statistical analysis; * P < 0.05, ** P < 0.01, and *** P < 0.001. Schematic images were created in Adobe Illustrator. FMO, fluorescence minus one.

Article Snippet: After red blood cell lysis (BioLegend), CRTH2 + cells were isolated using the CRTH2 MicroBead Kit (Miltenyi Biotec) as per the manufacturer’s protocol.

Techniques: Isolation, Cell Culture, Expressing, Quantitation Assay, Two Tailed Test, Fluorescence